In vitro tests for reproductive toxicity

Avantea uses a battery of in vitro tests for reproductive toxicity able of revealing adverse effects of chemicals and/or medical devices on specific reproductive processes. The tests are based on the application of assisted reproductive technologies, such as oocyte in vitro maturation, in vitro fertilization and early embryo development.

Bovine is the animal model used as the assisted reproductive technologies in this species are well developed, repeatable and widely applied in farm animals breeding. They can be used as a battery of tests in association with a more conventional citoxicity test (Alamar blue test) on somatic cells, in order to prove specific adverse reproductive effects as compared to unspecific effects.

In vitro maturation test (IVM):  it is a test which riproduces in vitro the oocyte maturation process. It corresponds to the in vivo pre-ovulatory phase and it allows to detect specific toxic effect on the oocyte.

In vitro fertilization test (IVF): this test involves the exposure of oocytes and spermatozoa to substances to be tested and it allows to evaluate the toxic effect both on the oocytes and on the oocyte-spermatozoa interaction during fertilization.

In vitro embryo culture test (IVC): this test is based on the use of bovine embryos one week after fertilization in order to evaluate toxic effect on the embryo development up to the hatched blastocyst stage, mimicking the beginning of the embryo development up to implantation.

Citoxicity test on 3T3 fibroblast cell line (Alamar blue assay): it allows to evaluate aspecific, non reproductive, toxic effects on mammalian cell proliferation and viability in vitro. This test has to be used in association wih IVM, IVF and IVC tests to differentiate generic toxicity from specific effect on reproductive processes.

In accordance with the 3Rs principle, in order to apply these assays it is not necessary to sacrifice animals as the oocytes are collected from ovaries of cows which have already been allocated in the food chain, whereas the bovine sperm is readly available on the market as frozen.

For more information on in vitro toxicity tests or to request a quote, contact us.

View and Download our Brochure